Study M562

Study name

Patterson E 2019

Title

Gamma-aminobutyric acid-producing lactobacilli positively affect metabolism and depressive-like behaviour in a mouse model of metabolic syndrome

Overall design

The aim of this study was to assess the efficacy of endogenous microbial GABA production in improving metabolic and behavioural outcomes in a mouse model of metabolic dysfunction. C57BL/6J mice were divided into the following 3 groups: (1) high fat diet group (high fat diet), (2) high fat diet + DPC6108 group (high fat diet and L. brevis DPC6108 treatment), (3) high fat diet + DSM32386 group (high fat diet and L. brevis DSM32386 treatment). High fat diet group received high fat diet for 12 weeks, and mice showed depressive-like behaviour compared with low fat diet group. Diet-induced obese and metabolically dysfunctional mice received one of two GABA-producing strains, L. brevis DPC6108 or L. brevis DSM32386, daily for 12 weeks. Ten plasma samples per group were used for metabonomic analysis.

Study Type

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Forced swimming test

Sample size

30

Tissue

Peripheral; Blood; Plasma;

Platform

MS-based; LC-MS: HPLC with ABI 4000 Q-Trap mass spectrometer (MDS Sciex);

PMID

31704943

DOI

10.1038/s41598-019-51781-x

Citation

Patterson E, Ryan PM, Wiley N, et al. Gamma-aminobutyric acid-producing lactobacilli positively affect metabolism and depressive-like behaviour in a mouse model of metabolic syndrome. Sci Rep. 2019;9(1):16323.

Metabolite

L-Arginine;

Ornithine;