Study M546
Study name | Huang YJ 2019 |
Title | PKA-mediated phosphorylation of CREB and NMDA receptor 2B in the hippocampus of offspring rats is involved in transmission of mental disorders across a generation |
Overall design | The aim of this study was to explore the mechanism of transmission of mental disorders across a generation. The effect of chronic unpredictable stress (CUS) before pregnancy on the behavioral response of adolescent offspring rats was examined. Female Sprague-Dawley rats were divided into the following 2 groups (n = 6 in each group): (1) control group, and (2) CUS group. The CUS stress procedure lasted for 3 weeks. When the CUS procedure was finished, all female rats were housed in pairs with a male rat for 1 week of mating. A total of 36 offspring from each group (3 females and 3 males from each mother) were randomly selected for the following experiments (CUS offspring group and control offspring group). Adolescent offspring rats in the CUS group exhibited depressive-like behavior. Magnetic resonance spectroscopy was used to measure the levels of glutamate, GABA, and glutamine in the hippocampus (n = 18), in order to identify changes by which pre-pregnancy stress could increase risk of behavioral disorders in adolescent offspring. Metabolite quantification was reported relative to total creatine as the reference peak. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 36 |
Tissue | Central; Brain; Hippocampus; |
Platform | MRS; MRS: MRI scanning (7T/160/AS small animal imaging system, Agilent Technologies, CA); |
PMID | |
DOI | |
Citation | Huang Y, Hu L, Li H, et al. PKA-mediated phosphorylation of CREB and NMDA receptor 2B in the hippocampus of offspring rats is involved in transmission of mental disorders across a generation. Psychiatry Res. 2019;280:112497. |
Metabolite | Glutamate/GABA ratio; Glutamate/Glutamine ratio; GABA/(Creatine and Phosphocreatine) ratio; |
