Study M501

Study name

Hill MN 2007

Title

Electroconvulsive shock treatment differentially modulates cortical and subcortical endocannabinoid activity

Overall design

To examine whether the endocannabinoid system is a potential target for the treatment of depression, the authors assessed the effects of electroconvulsive shock (ECS) treatment, both a single session and 10 daily sessions, on endocannabinoid content, CB(1) receptor binding parameters and CB(1) receptor-mediated [(35)S]GTPgammaS binding in the prefrontal cortex, hippocampus, hypothalamus and amygdala. Rats were divided into the following 3 groups: (1) control group (sham stimulation), (2) single ECS session group, (3) ten ECS sessions group. For analysis of endocannabinoid content, brain regions were subjected to a lipid extraction process. The contents of the two primary endocannabinoids anandamide and 2-arachidonylglycerol within lipid extracts were determined using isotope-dilution liquid chromatography-mass spectrometry.

Study Type

Type3;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Healthy individuals; Healthy individuals; Healthy individuals;

Criteria for depression

Not reported

Sample size

26

Tissue

Central; Brain; Hippocampus;

Central; Brain; Hypothalamus;

Central; Brain; Prefrontal cortex;

Central; Brain; Amygdala;

Platform

MS-based; LC-MS: not reported;

PMID

17561935

DOI

10.1111/j.1471-4159.2007.04688.x

Citation

Hill MN, Barr AM, Ho WS, et al. Electroconvulsive shock treatment differentially modulates cortical and subcortical endocannabinoid activity. J Neurochem. 2007;103(1):47-56.

Metabolite

Anandamide;