Study M490

Study name

Xin F 2018

Title

Mice exposed to bisphenol A exhibit depressive-like behavior with neurotransmitter and neuroactive steroid dysfunction

Overall design

The aim of this study was to investigate the molecular perturbations associated with endocrine disrupting chemical (EDC)-induced behavioral changes in first (F1) and second (F2) filial generations, using the model EDC bisphenol A (BPA). C57BL/6J dams were exposed to BPA from preconception until lactation through the diet at doses (10ug/kg bw/d-lower dose or 10mg/kg bw/d-upper dose) representative of human exposure levels. C57BL/6J female mice (F0 generation) were randomly assigned to control (control group) or BPA-containing diets (BPA group) beginning two weeks prior to mating. At least six F0 females per treatment group were exposed per cohort. Females were mated to unexposed C57BL/6J male mice during each cohort of exposure. All F1 generation offspring were weaned on postnatal day (PND) 21 and maintained on control diets throughout the remainder of the studies. To generate F2 offspring, a subset of six-week-old F1 females (one to two females per litter) were randomly selected to be mated to unexposed males. Animals used for adult behavior testing, aged 16-20 weeks, were randomly selected. The levels of testosterone, dehydroepiandrosterone (DHEA), and estrone in F1 adult male serum and whole hippocampal tissues were determined using liquid chromatography-high resolution mass spectrometry.

Study Type

Type1;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Forced swimming test

Sample size

40

Tissue

Central; Brain; Hippocampus;

Peripheral; Blood; Serum;

Platform

MS-based; LC-MS: not reported;

PMID

29763587

DOI

10.1016/j.yhbeh.2018.05.010

Citation

Xin F, Fischer E, Krapp C, et al. Mice exposed to bisphenol A exhibit depressive-like behavior with neurotransmitter and neuroactive steroid dysfunction. Horm Behav. 2018;102:93-104.

Metabolite

Dehydroepiandrosterone;