Study M352
Study name | Schulz D 2013 |
Title | Selective breeding for helplessness in rats alters the metabolic profile of the hippocampus and frontal cortex: a 1H-MRS study at 9.4 T |
Overall design | In this study, hydrogen-1 magnetic resonance spectroscopy at 9.4 T was used to measure the metabolic profiles of the hippocampus and frontal cortex in congenital learned helpless (cLH) and wild-type (WT) rats. The learned helplessness model of depression exposes animals to uncontrollable stress to induce changes in emotion, cognition and behaviour, but cLH rats were selectively bred to show changes in behaviour even without exposure to uncontrollable stress. Experimentally naive male 8- to 10-wk-old cLH (cLH group, n = 10) and WT rats (control group, n = 22) underwent spectroscopy and were exposed to uncontrollable stress 1 wk after the scan. Relative metabolite concentrations were calculated by taking the ratios of a particular metabolite over tNAA and tCr. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Learned helplessness model; Learned helplessness model; |
Criteria for depression | Active avoidance test |
Sample size | 32 |
Tissue | Central; Brain; Hippocampus; Central; Brain; Frontal cortex; Central; Brain; Prefrontal cortex; |
Platform | MRS; MRS: 9.4 T Biospec Avance 94/20AS MRI scanner (Bruker BioSpin MRI GmbH, Germany); |
PMID | |
DOI | |
Citation | Schulz D, Smith D, Yu M, et al. Selective breeding for helplessness in rats alters the metabolic profile of the hippocampus and frontal cortex: a 1H-MRS study at 9.4 T. Int J Neuropsychopharmacol 2013;16(1):199-212. |
Metabolite | Choline-containing compounds/(Creatine and Phosphocreatine) ratio; Glutamate/(Creatine and Phosphocreatine) ratio; Taurine/(Creatine and Phosphocreatine) ratio; Taurine/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; Glutamate/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; Phosphocreatine/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; Phosphocreatine/(Creatine and Phosphocreatine) ratio; Choline-containing compounds/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; Creatine/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; |
