Study M338
Study name | Choi JE 2017 |
Title | EPA and DHA, but not ALA, have antidepressant effects with 17beta-estradiol injection via regulation of a neurobiological system in ovariectomized rats |
Overall design | The purpose of the present study was to investigate the hypothesis that three major n-3 PUFAs, alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), individually had antidepressant effects combined with 17beta-estradiol-3-benzoate (E) through a neurobiological pathway in ovariectomized rats. Rats were fed a modified American Institute of Nutrition-93G diet with 0% n-3 PUFAs and 1% ALA, EPA and DHA relative to total energy intake for 12 weeks and were injected with corn oil or E every 4 days during the last 3 weeks. Rats were divided into the following 9 groups (n = 8 in each group): (1) sham group (control rats fed with 0% n-3 PUFA with sham surgery), (2) ovariectomy group (ovariectomized rats fed with 0% n-3 PUFA, without E injection), (3) E group (ovariectomized rats fed with 0% n-3 PUFA, with E injection), (4) ALA group (ovariectomized rats fed with 1% ALA, without E injection), (5) ALA + E group (ovariectomized rats fed with 1% ALA, with E injection), (6) EPA group (ovariectomized rats fed with 1% EPA, without E injection), (7) EPA + E group (ovariectomized rats fed with 1% EPA, with E injection), (8) DHA group (ovariectomized rats fed with 1% DHA, without E injection), and (9) DHA + E group (ovariectomized rats fed with 1% DHA, with E injection). After a week of acclimatization, all rats received one of these four isoenergetic experimental diets for 12 weeks. At week 8, rats underwent bilateral ovariectomy or sham surgery. From 1 week after the surgery, rats received corn oil (1 ml) with and without 10 ug of 17beta-estradiol-3-benzoate (E) subcutaneous injection every 4 days. Absolute metabolite concentrations were calculated using unsuppressed water signals. Metabolite quantification was reported relative to total creatine as the reference peak. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 72 |
Tissue | Central; Brain; Hippocampus; |
Platform | MRS; MRS: 7.0-T/160-mm PharmaScan system (Bruker Biospin, Ettlingen, Germany); |
PMID | |
DOI | |
Citation | Choi JE, Park Y. EPA and DHA, but not ALA, have antidepressant effects with 17beta-estradiol injection via regulation of a neurobiological system in ovariectomized rats. J Nutr Biochem 2017;49:101-9. |
Metabolite | myo-Inositol/(Creatine and Phosphocreatine) ratio; |
