Study M292
Study name | Biedermann S 2012 |
Title | Increase of hippocampal glutamate after electroconvulsive treatment: a quantitative proton MR spectroscopy study at 9.4 T in an animal model of depression |
Overall design | The aim of this study was to perform quantification of hippocampal metabolites with proton magnetic resonance spectroscopy (1H MRS) in the congenital learned helpless (cLH) animal model of depression in order to determine the role of hippocampal metabolites in the development of depressive-like behaviour and to assess the effects of electroconvulsive shocks (ECS). A well-validated animal model of treatment resistant depression (cLH) was investigated by hippocampal in vivo 1H MRS with and without a 1-week course of ECS, an animal model of ECT, and compared to wild type (WT) animals, while saline and clomipramine injections served as additional controls. Rats were divided into the following 6 groups (n = 6 in each group): (1) control group (wildtype rats and no ECS treatment), (2) cLH group (cLH rats), (3) ECS group (wildtype rats treated with a 5-day course of daily-administered ECS), (4) cLH + ECS group (cLH rats treated with a 5-day course of daily-administered ECS), (5) clomipramine group (wildtype rats treated with clomipramine at the dose of 10 mg/kg i.p. for 5 days), (6) saline group (wildtype rats treated with saline injections). Metabolite quantification was reported in absolute concentrations. |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; Animal model; Learned helplessness model; Learned helplessness model; |
Criteria for depression | Active avoidance test |
Sample size | 36 |
Tissue | Central; Brain; Hippocampus; |
Platform | MRS; MRS: 9.4 T animal MR scanner (Bruker, Rheinstetten, Germany); |
PMID | |
DOI | |
Citation | Biedermann S, Weber-Fahr W, Zheng L, et al. Increase of hippocampal glutamate after electroconvulsive treatment: a quantitative proton MR spectroscopy study at 9.4 T in an animal model of depression. World J Biol Psychiatry 2012;13(6):447-57. |
Metabolite | L-Glutamic acid; D-Glucose; Taurine; Choline-containing compounds; Glutamate/(Creatine and Phosphocreatine) ratio; Taurine/(Creatine and Phosphocreatine) ratio; Taurine/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; Glucose(Creatine and Phosphocreatine) ratio; Glucose/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; Glutamate/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; |
