Study M276
Study name | Kim SY 2010a |
Title | Reversal of myo-inositol metabolic level in the left dorsolateral prefrontal cortex of rats exposed to forced swimming test following desipramine treatment: an in vivo localized 1H-MRS study at 4.7 T |
Overall design | The purpose of this study was to assess desipramine (DMI) effects on the left dorsolateral prefrontal cortex (DLPFC) of the rats, which were randomly assigned to three groups (control, FST + saline, FST + DMI), using single-voxel localization technique. Rats were divided into the following 3 groups (n = 10 in each group): (1) control group, (2) FST group, (3) FST + DMI group. The DMI treatment (10 mg/kg) was administered with a subchronic pattern. Three injections were administered at 23, 5 and 1 h prior to the test session of forced swimming test. All 1H-MRS experiments were performed on a Bruker 4.7-T scanner with 400 mm bore magnet, allowing for acquisition of in vivo 1H point-resolved spectroscopy spectra. Proton metabolites were quantified automatically using LCModel software and were expressed as ratios to total creatine (Cr + PCr). Major target metabolites such as N-acetyl aspartate (NAA) + N-acetylaspartylglutamate (NAAG), glutamate + glutamine (Glu + Gln), glycerophosphorylcholine + phosphorylcholine (GPC + PCho), myo-inositol (mIns) and taurine (Tau) were successfully quantified with Cramer-Rao lower boundary <= 10%. In vivo MRI/MRS scanning was performed 1 h later after forced swimming test. Only data from FST group and FST + DMI group was used. Metabolite quantification was reported relative to total creatine as the reference peak. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 20 |
Tissue | Central; Brain; Dorsolateral prefrontal cortex; |
Platform | MRS; MRS: 4.7 T BIOSPEC scanner (Bruker Medical GmbH, Ettlingen, Germany); |
PMID | |
DOI | |
Citation | Kim SY, Choi CB, Lee HS, et al. Reversal of myo-inositol metabolic level in the left dorsolateral prefrontal cortex of rats exposed to forced swimming test following desipramine treatment: an in vivo localized 1H-MRS study at 4.7 T. Magn Reson Imaging 2010;28(10):1461-7. |
Metabolite | myo-Inositol/(Creatine and Phosphocreatine) ratio; Myo-inositol/(N-Acetyl aspartate and N-Acetylglutamic acid) ratio; |
