Study M197

Study name

Hill MN 2005

Title

Downregulation of endocannabinoid signaling in the hippocampus following chronic unpredictable stress

Overall design

The aim of this study was to investigate whether alterations in endogenous cannabinoid (eCB) signaling might contribute to the cognitive impairments induced by chronic stress. Seventy-day-old male Long-Evans rats were used in this study. Rats were divided into the following 2 groups: (1) control group, (2) CUS group (chronic unpredictable stress paradigm). Five samples per group were used for LC-MS analysis. The CUS stress procedure lasted for 3 weeks. The content of both 2-arachidonylglycerol (2-AG) and anandamide (AEA) in hippocampus and limbic forebrain were determined using isotope-dilution liquid chromatography/mass spectrometry.

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Long-Evans rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Not reported

Sample size

10

Tissue

Central; Brain; Limbic forebrain;

Central; Brain; Hippocampus;

Platform

MS-based; LC-MS: not reported;

PMID

15525997

DOI

10.1038/sj.npp.1300601

Citation

Hill MN, Patel S, Carrier EJ, et al. Downregulation of endocannabinoid signaling in the hippocampus following chronic unpredictable stress. Neuropsychopharmacology 2005;30(3):508-15.

Metabolite

MG(0:0/20:4(5Z,8Z,11Z,14Z)/0:0);