Study M196
Study name | Selley ML 2004 |
Title | Increased (E)-4-hydroxy-2-nonenal and asymmetric dimethylarginine concentrations and decreased nitric oxide concentrations in the plasma of patients with major depression |
Overall design | The aim of this study was to determine plasma (E)-4-Hydroxy-2-nonenal (HNE), asymmetric dimethylarginine (ADMA) and nitric oxide (NO) concentrations in patients with major depression compared to normal volunteers and to examine the effect of HNE on ADMA formation and dimethylarginine dimethylaminohydrolase (DDAH) activity in cultured endothelial cells. The study was conducted in patients with major depression (depression group, n = 25) and healthy control subjects (control group, n = 25). Plasma concentrations of HNE were determined as the Opentafluorylbenzyl oxime using capillary column GC-MS and deuterated HNE as the internal standard; ADME by LC-MS-MS using 13C6-L-arginine as the internal standard; and NO by GC-MS following reduction to nitrate and nitrite and derivatisation to the pentafluorobenzyl derivative using [15N]nitrate and [15N]nitrite as the internal standards. The patients were anti-depressant naive. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Human; |
Categories of depression | Depressive disorder; Depression; Depression; |
Criteria for depression | DSM-IV diagnosed MDD |
Sample size | 50 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: not reported; MS-based; GC-MS: not reported; |
PMID | |
DOI | |
Citation | Selley ML. Increased (E)-4-hydroxy-2-nonenal and asymmetric dimethylarginine concentrations and decreased nitric oxide concentrations in the plasma of patients with major depression. J Affect Disord 2004;80(2-3):249-56. |
Metabolite |
