Study M191

Study name

Chandrasekhar BVS 2017

Title

A focus on the effect of L-theanine on improving depression and cognition in C57BL/J male mice subjected for chronic stress induced neuroinflammation

Overall design

The aim of this study was to elucidate the effect and mechanism of L-theanine on chronic restrainer stress (CRS) induced neuroinflammation, anxiety-like, depression, learning and memory impairment in C57BL/J male mice. Chronic restrain stress was induced by well ventilated 50 ml conical tube for 6 hours/day 21 consecutive days. After stressor cessation behavioral and recognition tests were conducted, at the end of study mice were sacrificed, brain and blood was collected. Cytokines (TNF-alpha and IL-6), neurotransmitters were quantified in prefrontal cortex and estimated corticosterone levels in plasma. Mice were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CRS group, and (3) CRS + L-theanine group (stressor plus L-theanine treatment at the dose of 4 mg/kg). In CRS procedure, mice were restrained in falcon tube inside well-ventilated and 6 h/day for three weeks, and L-theanine was administered orally once per day during the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/J mouse;

Categories of depression

Animal model; Chronic restraint stress model; Chronic restraint stress model;

Criteria for depression

Forced swimming test

Sample size

24

Tissue

Central; Brain; Prefrontal cortex;

Platform

MS-based; LC-MS: Shimadzu LCMS 8040 triple quadrupole system with UHPLC (NEXERA);

PMID

N/A

DOI

10.13005/bpj/1199

Citation

Chandrasekhar BVS, Elango P, Maheswari SU, et al. A focus on the effect of L-theanine on improving depression and cognition in C57BL/J male mice subjected for chronic stress induced neuroinflammation. Biomed Pharma J 2017, 10(2):1015-27.

Metabolite

Serotonin;

Norepinephrine;