Study M179

Study name

Jiang N 2017

Title

[Antidepressant effects of the extract of Dendrobium nobile Lindl on chronic unpredictable mild stress-induced depressive mice]

Overall design

The aim of this study was to investigate whether the extract of Dendrobium nobile Lindl (DNL) has an antidepressant effect on chronic unpredictable mild stress (CUMS)-induced depressive mice. Mice were divided into the following 6 groups (n = 12 in each group): (1) control group, (2) CUMS group, (3) CUMS + paroxetine group (stressor plus paroxetine treatment at the dose of 10 mg/kg), (4) CUMS + low dose of DNL group (stressor plus extract of Dendrobium nobile Lindl treatment at the dose of 50 mg/kg), (5) CUMS + middle dose of DNL group (stressor plus Dendrobium nobile Lindl treatment at the dose of 100 mg/kg), and (6) CUMS + high dose of DNL group (stressor plus Dendrobium nobile Lindl treatment at the dose of 200 mg/kg). The levels of dopamine (DA) and 5-hydroxytryptamine (5-HT) in cerebral cortex and hippocampus tissues were measured by the liquid chromatography-mass spectrometer (LC-MS)/MS. Eight samples per group were used for LC-MS analysis. The CUMS stress procedure lasted for 5 weeks, and drugs were administered orally once daily for 8 weeks (containing two-week preventive medication before the modeling).

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; BALB/c mouse;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test, tail suspension test

Sample size

48

Tissue

Central; Brain; Hippocampus;

Central; Brain; Cerebral cortex;

Platform

MS-based; LC-MS: Agilent 1200 series HPLC (Agilent Technologies, Inc., USA) with Q-Trap5500 mass spectrometer Q-Trap5500 (Applied Biosystems, USA);

PMID

28435974

DOI

10.13294/j.aps.2017.0006

Citation

Jiang N, Fan LX, Yang YJ, et al. [Antidepressant effects of the extract of Dendrobium nobile Lindl on chronic unpredictable mild stress-induced depressive mice]. Sheng Li Xue Bao 2017;69(2):159-66. (Article in Chinese)

Metabolite

Serotonin;

Dopamine;