Study M169
Study name | Zhai XJ 2015b |
Title | A simple LC-MS/MS method for the determination of cortisol, cortisone and tetrahydro-metabolites in human urine: assay development, validation and application in depression patients |
Overall design | To better understand depression a LC-MS/MS method for simultaneous determination of cortisol (F), cortisone (E), tetrahydrocortisol (THF), allo-tetrahydrocortisol (5alpha-THF) and tetrahydrocortisone (THE) in human urine has been developed and validated. The nocturnal urine concentrations of F, E and tetrahydro-metabolites in apparently healthy male adult volunteers (control group, n = 12) and drug-free male patients with a diagnosis of depression (depressed group, n = 12) were analyzed. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Human; |
Categories of depression | Depressive disorder; Depression; Depression; |
Criteria for depression | Not reported |
Sample size | 24 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; LC-MS: 1200 series LC system (Agilent, Santa Clara, CA, USA) with AB Sciex Qtrap 4000 tandem mass spectrometer (Foster City, CA, USA); |
PMID | |
DOI | |
Citation | Zhai X, Chen F, Zhu C, et al. A simple LC-MS/MS method for the determination of cortisol, cortisone and tetrahydro-metabolites in human urine: assay development, validation and application in depression patients. J Pharm Biomed Anal 2015;107:450-5. |
Metabolite | Cortisol; Tetrahydrocortisol; Cortisol/Cortisone ratio; (Tetrahydrocortisol + 5alpha-Tetrahydrocortisol)/Tetrahydrocortisone; 5a-Tetrahydrocortisol; |
