Study M145
Study name | Huang F 2014 |
Title | Simultaneous quantification of seven hippocampal neurotransmitters in depression mice by LC-MS/MS |
Overall design | There is no method available to simultaneously detect GABA, Glu, Epi, NE, DA, 5-HT and 5-HIAA in mouse hippocampus. In this study, a rapid and sensitive LC-MS/MS method has been developed for simultaneously measuring seven neurotransmitters in mouse hippocampus. The mouse were divided into the following 2 groups: (1) control group (PBS intraperitoneally; n = 8), (2) model group (i.p. with 50 mg/kg methylprednisolone consecutively for 3 weeks; n = 12). |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 20 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Agilent 1290 HPLC system with Agilent 6410 triple stage quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA, USA); |
PMID | |
DOI | |
Citation | Huang F, Li J, Shi HL, et al. Simultaneous quantification of seven hippocampal neurotransmitters in depression mice by LC-MS/MS. J Neurosci Methods 2014;229:8-14. |
Metabolite |
