Study M142
Study name | Zhou WJ 2015 |
Title | Peripheral indoleamine 2,3-dioxygenase 1 is required for comorbid depression-like behavior but does not contribute to neuropathic pain in mice |
Overall design | Chronic pain frequently co-occurs with major depressive disorder but the mechanisms are poorly understood. The authors investigated the contribution of indoleamine 2,3-dioxygenase-1 (IDO1), a rate-limiting enzyme in the conversion of tryptophan to neurotoxic metabolites, to this comorbidity using the spared nerve injury (SNI) model of neuropathic pain in mice. SNI resulted in unilateral mechanical allodynia, reduced social interaction, and increased immobility in the forced swim test without changes in locomotor activity. Assessment of brain and plasma metabolites was conducted using HPLC-MS. Mice were divided into 2 groups: (1) control group (sham surgery), and (2) SNI group (spared nerve injury model of neuropathic pain). For SNI surgery, the sural common peroneal and tibial branches of the left sciatic nerve were exposed under isoflurane anesthesia. The tibial and common peroneal nerves were transected. The sural nerve was kept intact. Forced swim test was performed on day 7 after SNI surgery. On day 8, mice were sacrificed for tissue collection. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57/BL6J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 15 |
Tissue | Peripheral; Blood; Plasma; Central; Brain; Hippocampus; Central; Brain; Frontal cortex; |
Platform | MS-based; LC-MS: Waters Acquity HPLC system with Waters Quattro Premier XE triple quadrupole mass spectrometer; |
PMID | |
DOI | |
Citation | Zhou W, Dantzer R, Budac DP, et al. Peripheral indoleamine 2,3-dioxygenase 1 is required for comorbid depression-like behavior but does not contribute to neuropathic pain in mice. Brain Behav Immun 2015; 46:147-53. |
Metabolite |
