Study M141

Study name

Qian Z 2016

Title

Effects of Jiaotaiwan on depressive-like behavior in mice after lipopolysaccharide administration

Overall design

This study is to determine whether Jiaotaiwan (JTW) was benefit for the treatment of lipopolysaccharide (LPS)-induced depression-like behavior in mice and explore its possible mechanism. Mice were divided into 5 groups (n = 20 in each group): (1) control group, (2) LPS group (LPS-induced group, 0.9 % physiological saline, 10 ml/kg), (3) LPS + fluoxetine group (LPS plus fluoxetine treatment at the dose of 20 mg/kg), (4) LPS + low dose of JTW group (stress plus Jiaotaiwan treatment at the dose of 4.2 g/kg), and (5) CUMS + high dose of JTW group (stress plus Jiaotaiwan treatment at the dose of 8.4 g/kg). All drugs were intragastrically administered once daily for 7 consecutive days. On the 7th day, LPS (0.5 mg/kg, i.p.) was injected into mice 30 min after drug administration. Behavioral tests were performed 24 h after LPS administration. The 5-hydroxytryptamine and nor-epinephrine levels in prefrontal cortex were determined by UPLC-MS. Ten sample per group were used for metabonomic analysis.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; ICR mouse;

Categories of depression

Animal model; Lipopolysaccharide induced depression model; Lipopolysaccharide induced depression model;

Criteria for depression

Sucrose preference test, forced swimming test, tail suspension test

Sample size

50

Tissue

Central; Brain; Prefrontal cortex;

Platform

MS-based; LC-MS: Waters ACQUITY UPLC system with Waters ACQUITY triple-quadrupole tandem mass spectrometer (Waters Corp., Milford, MA, USA);

PMID

27796565

DOI

10.1007/s11011-016-9925-8

Citation

Zhe Q, Sulei W, Weiwei T, et al. Effects of Jiaotaiwan on depressive-like behavior in mice after lipopolysaccharide administration. Metab Brain Dis 2017; 32(2):415-26.

Metabolite

Serotonin;

Norepinephrine;