Study M128

Study name

Sun SQ 2015

Title

Reduced cholesterol is associated with the depressive-like behavior in rats through modulation of the brain 5-HT1A receptor

Overall design

In current study, rats were exposed to chronic mild stress (CMS) for consecutive 28 days, and the depressive-like behavior was tested by sucrose preference test, immobility in the forced swim test, locomotor activity in the open field test, decreased bodyweight and food intake. Additionally, the total cholesterol levels in the medial prefrontal cortex (mPFC) and the hippocampus of rats were measured by gas chromatograph mass spectrometer. In Experiment 1, the rats were divided into 2 groups (n = 8 in each group): (1) control group, (2) CMS group. Medial prefrontal cortex (mPFC) and hippocampus were collected for further cholesterol assay. The total cholesterol levels were measured by gas chromatograph mass spectrometer. The CMS stress procedure lasted for 4 weeks, and cholesterol were administered during the model building period (1 g/kg, experimental diet).

Study Type

Type1;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test, forced swimming test

Sample size

16

Tissue

Central; Brain; Medial prefrontal cortex;

Central; Brain; Hippocampus;

Platform

MS-based; GC-MS: gas chromatograph mass spectrometer;

PMID

25889773

DOI

10.1186/s12944-015-0020-7

Citation

Sun S, Yang S, Mao Y, et al. Reduced cholesterol is associated with the depressive-like behavior in rats through modulation of the brain 5-HT1A receptor. Lipids Health Dis 2015;14:22.

Metabolite

Cholesterol;