Study M122
Study name | Lee LHW 2009 |
Title | Lipidomic analyses of the mouse brain after antidepressant treatment: evidence for endogenous release of long-chain fatty acids? |
Overall design | The present study aims to examine effects of antidepressants on lipids in different regions of the brain at individual molecular species level, using the novel technique of lipidomics. The prefrontal cortex, hippocampus, striatum and cerebellum were harvested, and lipid profiles compared to those of saline-injected mice using high-performance liquid chromatography/mass spectrometry (HPLC/MS). Mice were divided into 4 groups (n = 4 in each group): (1) control group (saline), (2) maprotiline group (maprotiline treatment at the dose of 10 mg/kg), (3) fluoxetine group (fluoxetine treatment at the dose of 10 mg/kg), (4) paroxetine group (paroxetine treatment at the dose of 10 mg/kg). Drugs were intraperitoneal administered daily for 4 weeks. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; BALB/c mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | 16 |
Tissue | Central; Brain; Striatum; Central; Brain; Cerebellum; Central; Brain; Prefrontal cortex; Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Agilent HPLC system coupled with Triple Quadrupole/Ion Trap mass spectrometer (4000 Q trap, Applied Biosystems, USA); |
PMID | |
DOI | |
Citation | Lee LH, Shui G, Farooqui AA, et al. Lipidomic analyses of the mouse brain after antidepressant treatment: evidence for endogenous release of long-chain fatty acids? Int J Neuropsychopharmacol 2009;12(7):953-64. |
Metabolite | LysoPC(16:0); LysoPC(18:0); LysoPC(18:1); LysoPC(18:2); PC(38:4); PC(38:6); PC(40:6); PE(34:2); LysoPC(16:1/0:0); PC(32:0); PC(36:3); PE(38:5); Cer(d18:1/24:0); LysoPC(20:4); PC(30:0e); PC(30:0); PC(36:3p); SM(18/16:0); Cer(d18:1/16:0); Cer(d18:1/18:0); Cer(d18:1/20:0); Cer(d18:1/22:0); PI(38:6); PI(40:4); Cer(d18:1/24:1); PI(40:6); PC(36:1); PC(38:3); PC(40:2p); |
