Study M1135

Study name

Cai W 2023

Title

Effects of electroacupuncture on gut microbiota and fecal metabolites in rats with poststroke depression

Overall design

The aim of this study was to investigate the effects of electroacupuncture on alterations in fecal metabolome in poststroke depression (PSD) rats. Sprague-Dawley rats were divided into the following 3 groups: (1) control group, (2) PSD group, and (3) PSD + electroacupuncture group. For the PSD groups, transient middle cerebral artery occlusion surgery was performed, then rats were subjected to chronic unpredictable mild stress (CUMS) for 5 weeks after surgery. Electroacupuncture was administered during the last 2 weeks of the model building period. Fecal metabolomic analysis was performed using LC-MS (n=8 per group).

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Sucrose preference test

Sample size

24

Tissue

Peripheral; Faece; Faece;

Platform

MS-based; LC-MS: UHPLC-Q Exactive HF-X system with Thermo UHPLC-Q Exactive HF-X mass spectrometer (Thermo Fisher Scientifc);

PMID

37465562

DOI

10.2147/NDT.S415098

Citation

Cai W, Wei XF, Hu C, et al. Effects of electroacupuncture on gut microbiota and fecal metabolites in rats with poststroke depression. Neuropsychiatr Dis Treat. 2023 Jul 12;19:1581-1592.

Metabolite

Citric acid;

L-Glutamic acid;

Indoleacetic acid;

5-Methoxytryptophol;

Citrulline;

Deoxyguanosine;

Asymmetric dimethylarginine;

Acetylcholine;

LysoPC(20:4/0:0);

5a-Pregnane-3,20-dione;

Tetrahydrodeoxycorticosterone;

1,3,7,12-Tetrahydroxycholan-24-oic acid;

Adenosine diphosphate ribose;

N-Acetylputrescine;

Isoleucyl-Phenylalanine;

1-Pyrroline-5-carboxylic acid;

12a-Hydroxy-3-oxocholadienic acid;

N,O-Didesmethylvenlafaxine;

5,7-dihydroxy-2-(1-hydroxy-3,5-dimethoxy-4-oxocyclohex-2-en-1-yl)-4H-chromen-4-one;

4-Vinylphenol sulfate;

Estradiol;

3b-Hydroxy-5-cholenoic acid;

PC(20:4/0:0);

N1,N12-Diacetylspermine;

2-(2-Aminoethyl)indole;

Deuteroporphyrin IX;

Methyl ricinoleate;

10-Hydroxymelleolide;

Malvidin;

11alpha-(chloromethyl)-1alpha,25-dihydroxyvitamin D3;

(ent-16betaOH)-16,17-Dihydroxy-9(11)-kauren-19-oic acid;

5',8-Dihydroxy-3',4',7-trimethoxyflavan;

(-)-Folicanthine;

(ent-6alpha,7alpha,12alpha)-6,7,12-Trihydroxy-16-kauren-19-oic acid;

Diketopiperazine derivative of Quinaprilat;

11-Dodecenoic acid;

Kojibiose;

(12S,15S)-15-O-Demethyl-10,29-dideoxy-11,12-dihydro-striatin C;

Thr Cys Asn Glu;

(S)-beta-Aminoisobutyric acid;

Asteltoxin;

Trichocarposide;

1,2-Diphenyl-3,5-dioxo-4-(3-hydroxybutyl)pyrazolidine;

2-Methoxycinnamic acid;

(±)-Aegeline;

4-p-Coumaroyl-1,5-quinolactone;

cis-Resveratrol 3-O-glucuronide;

20-Hydroxy-leukotriene E4;

2-Hydroxyestradiol;

3,4,5-trihydroxy-6-{3-hydroxy-4-[(E)-2-[2,3,5-trihydroxy-4-(3-methylbut-2-en-1-yl)phenyl]ethenyl]phenoxy}oxane-2-carboxylic acid;

3-Methylthiopropanamine;

2-amino-4-({1-[(carboxymethyl)-C-hydroxycarbonimidoyl]-2-{[1-(2-hydroxyphenyl)-3-oxopropyl]sulfanyl}ethyl}-C-hydroxycarbonimidoyl)butanoic acid;

Cis-2,3-Dimethylthiirane;

Melledonal C;

5alpha-Pregnan-20alpha-ol-3-one;

Valylisoleucine;

Safynol;

Securinine;

alpha-Pyrrolidinovalerophenone;

Cotarnine;

(R)-Sulcatol;

Aminoethoxyacetic acid;

4-Methylene-L-glutamine;

11-peroxy-5Z,8Z,12E,14Z-eicosatetraenoate;

Yucalexin B22;

Arnidenediol;

Cardanolmonoene;

5,20-DiHETE;

TetraHCA;

Isobutyl 2-furanpropionate;