Study M1125
Study name | Ikeda Y 2023 |
Title | Direct evidence for the involvement of intestinal reactive oxygen species in the progress of depression via the gut-brain axis |
Overall design | The aim of this study was to elucidate the impact of scavenging intestinal reactive oxygen species on depression treatment via the gut-brain axis in the chronic restraint stress (CRS) model. A novel polymer-based antioxidant (siSMAPoTN), which was distributed only in the intestine and did not diffuse into the whole body after oral administration, was used. c57BL/6J mice were divided into the following 3 groups (n = 8 in each group): (1) control group, (2) CRS group, and (3) CRS + siSMAPoTN group. The CRS stress procedure lasted for 3 weeks. The siSMAPoTN group was provided ad libitum access to a nanoparticle solution (650 mg-polymer/kg; 5 mmol TEMPO/L) instead of water. Plasma corticosterone levels were evaluated using an LC-MS/MS system. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; c57BL/6J mouse; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 24 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: LaChrom Ultra (Hitachi, Japan) with API 2000 (AB SCIEX, Canada); |
PMID | |
DOI | |
Citation | Ikeda Y, Saigo N, Nagasaki Y. Direct evidence for the involvement of intestinal reactive oxygen species in the progress of depression via the gut-brain axis. Biomaterials. 2023 Apr;295:122053. |
Metabolite |
