Study M1121
Study name | Sun N 2022b |
Title | Design of fast-onset antidepressant by dissociating SERT from nNOS in the DRN |
Overall design | The aim of this study was to examine whether dissociating serotonin transporter (SERT) from neuronal nitric oxide synthase (nNOS) in the dorsal raphe nucleus (DRN) could exert a fast-onset antidepressant effect. In this study, a peptide named TAT-SERT-15C was synthesized, in which the 15 C-terminal amino acids of SERT (SERT-15C) were N-terminally fused to the transduction domain of the Tat protein from the HIV type 1. C57BL/6J mice were divided into the following 2 groups: (1) control group (vehicle treatment), and (2) TAT-SERT-15C group (TAT-SERT-15C treatment at the dose of 1.6 ug). TAT-SERT-15C was microinjected into the DRN of mice, which produced an antidepressant-like effect. The levels of serotonin, noradrenalin, and dopamine in the DRN were determined at 2 hours using in vivo microdialysis combined with liquid chromatography-mass spectrometry (n=5/group). |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test, tail suspension test |
Sample size | 10 |
Tissue | Central; Brain; Dorsal raphe nucleus; |
Platform | MS-based; LC-MS: not reported; |
PMID | |
DOI | |
Citation | Sun N, Qin YJ, Xu C, et al. Design of fast-onset antidepressant by dissociating SERT from nNOS in the DRN. Science. 2022 Oct 28;378(6618):390-398. |
Metabolite |
