Study M1115

Study name

Li D 2023c

Title

Gut-microbiome-expressed 3beta-hydroxysteroid dehydrogenase degrades estradiol and is linked to depression in premenopausal females

Overall design

The aim of this study was to determine whether K. aerogenes TS2020 can induce depression-like behaviors in female mice. K. aerogenes-recipient mice was administered with cefotaxime to investigate whether serum estradiol reduction and depression-like behavior in the mice could be alleviated by antibiotic treatment. C57BL/6J mice were divided into the following 3 groups: (1) ovariectomy + estradiol group (ovariectomy followed by gavage estradiol for 6 weeks), (2) ovariectomy + estradiol + K. aerogenes TS2020 group (ovariectomy followed by gavage estradiol for 6 weeks and K. aerogenes TS2020 for 4 weeks), and (3) ovariectomy + estradiol + K. aerogenes TS2020 + cefotaxime group (ovariectomy followed by gavage estradiol for 6 weeks and K. aerogenes TS2020 for 4 weeks, then treated with cefotaxime for 1 week). The results of behavioral assessments showed that K. aerogenes TS2020 can induce depressive-like behaviors, and cefotaxime administration can alleviate such depressive-like behaviors in mice. Liquid chromatography-tandem mass spectrometry was used to compare serum estradiol levels between groups (n = 6/group).

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Forced swimming test, tail suspension test

Sample size

18

Tissue

Peripheral; Blood; Serum;

Platform

MS-based; LC-MS: Ekspert ultraLC 100-XL system with AB SCIEX 4500 QTRAP mass spectrometer (Applied Biosystems, Foster City, CA, USA);

PMID

36933555

DOI

10.1016/j.cmet.2023.02.017

Citation

Li D, Sun T, Tong Y, et al. Gut-microbiome-expressed 3beta-hydroxysteroid dehydrogenase degrades estradiol and is linked to depression in premenopausal females. Cell Metab. 2023 Apr 4;35(4):685-694.e5.

Metabolite

Estradiol;