Study M1114

Study name

Li D 2023b

Title

Gut-microbiome-expressed 3beta-hydroxysteroid dehydrogenase degrades estradiol and is linked to depression in premenopausal females

Overall design

The aim of this study was to explore whether estradiol-degrading microbes in the guts of female patients are linked to depression. C57BL/6J female mice were divided into the following 5 groups: (1) control group (sham ovariectomy), (2) ovariectomy group (ovariectomy), (3) ovariectomy + estradiol group (ovariectomy followed by gavage estradiol for 6 weeks), (4) ovariectomy + estradiol + depression microbiota group (ovariectomy followed by gavage estradiol for 6 weeks and depression microbiota for 4 weeks), and (5) ovariectomy + estradiol + non-depression microbiota group (ovariectomy followed by gavage estradiol for 6 weeks and non-depression microbiota for 4 weeks). Depression and non-depression microbiota were isolated from feces of individuals with and without depression. Liquid chromatography-tandem mass spectrometry was used to compare serum estradiol levels between groups.

Study Type

Type1;

Data available

Unavailable

Organism

Mouse; C57BL/6J mouse;

Categories of depression

Animal model; Other animal model; Other animal model;

Criteria for depression

Forced swimming test, tail suspension test

Sample size

47

Tissue

Peripheral; Blood; Serum;

Platform

MS-based; LC-MS: Ekspert ultraLC 100-XL system with AB SCIEX 4500 QTRAP mass spectrometer (Applied Biosystems, Foster City, CA, USA);

PMID

36933555

DOI

10.1016/j.cmet.2023.02.017

Citation

Li D, Sun T, Tong Y, et al. Gut-microbiome-expressed 3beta-hydroxysteroid dehydrogenase degrades estradiol and is linked to depression in premenopausal females. Cell Metab. 2023 Apr 4;35(4):685-694.e5.

Metabolite

Estradiol;