Study M111
Study name | Martin AE 2017 |
Title | Further evaluation of mechanisms associated with the antidepressant-like signature of scopolamine in mice |
Overall design | Male, wildtype (C57BL/6) and muscarinic receptor M1-null mice were generated. Scopolamine (3 mg/kg, i.p.) or vehicle (water) were administered to M1+/+ or M1-/- mice. Mice were divided into 4 groups: (1) WT-Veh group (M1+/+ wild type genotype treated with vehicle), (2) WT-Scop group (M1+/+ wild type genotype treated with single dose of scopolamine 3 mg/kg, i.p.), (3) KO-Veh group (M1-/- knockout genotype treated with vehicle), (4) KO-Scop group (M1-/- knockout genotype treated with single dose of scopolamine 3 mg/kg). Plasma and frontal cortex were obtained at 30 min post dosing from these mice for analysis. Analysis was accomplished utilizing non-targeted and targeted profiling using GC-TOF-MS and HILIC-LC-MS/MS. Only data from wild type genotype mice were used. |
Study Type | Type3; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Not reported |
Sample size | N/A |
Tissue | Peripheral; Blood; Plasma; Central; Brain; Frontal cortex; |
Platform | MS-based; GC-MS: Agilent 7890A gas chromatograph (Agilent, Palo Alto, CA, USA) interfaced to a high resolution time-of-flight Pegasus GC-HRT mass spectrometer (Leco, St. Joseph, MI, USA); MS-based; LC-MS: NEXERA UPLC system (Shimadzu, USA) coupled to Triple Quad 5500 System (AB Sciex, Framingham, MA, USA); |
PMID | |
DOI | |
Citation | Martin AE, Schober DA, Nikolayev A, et al. Further evaluation of mechanisms associated with the antidepressant-like signature of scopolamine in mice. CNS Neurol Disord Drug Targets 2017;16(4):492-500. |
Metabolite |
