Study M1099
Study name | Filipovic D 2023 |
Title | Metabolic fingerprints of effective fluoxetine treatment in the prefrontal cortex of chronically socially isolated rats: marker candidates and predictive metabolites |
Overall design | The aim of this study was to investigate the application of liquid chromatography-high resolution mass spectrometry-based untargeted metabolomics in chronic social isolation rats with chronic fluoxetine treatment. Wistar rats were divided into the following 4 groups (n= 6-8 in each group): (1) control group, (2) chronic social isolation group, (3) fluoxetine group (non-stress with fluoxetine treatment at the dose of 15 mg/kg), and (4) chronic social isolation + fluoxetine group (stress with fluoxetine treatment at the dose of 15 mg/kg). The chronic social isolation stress procedure lasted for 6 weeks, and fluoxetine was administered intraperitoneally once per day during the last 3 weeks of the model building period. Untargeted metabolomics of the prefrontal cortex of rats were carried out using liquid chromatography-high resolution mass spectrometry. |
Study Type | Type1; Type2; Type3; |
Data available | |
Organism | Rat; Wistar rat; |
Categories of depression | Healthy individuals; Healthy individuals; Healthy individuals; Animal model; Other animal model; Other animal model; |
Criteria for depression | Forced swimming test |
Sample size | 27 |
Tissue | Central; Brain; Prefrontal cortex; |
Platform | MS-based; LC-MS: Dionex Ultimate 3000 RS LC-system with Orbitrap mass spectrometer (QExactive, ThermoFisher Scientific, Bremen, Germany); |
PMID | |
DOI | |
Citation | Filipovic D, Inderhees J, Korda A, et al. Metabolic fingerprints of effective fluoxetine treatment in the prefrontal cortex of chronically socially isolated rats: marker candidates and predictive metabolites. Int J Mol Sci 2023;24,10957. |
Metabolite | myo-Inositol; Indoxyl sulfate; Xanthine; Hypotaurine; Xanthosine; L-Acetylcarnitine; D-Sedoheptulose 7-phosphate; Riboflavin; |
