Study M108
Study name | Lei TL 2017 |
Title | A comparative study of the main constituents and antidepressant effects of raw and vinegar-baked Bupleuri Radix in rats subjected to chronic unpredictable mild stress |
Overall design | The present study identified the main chemical compositions in Bupleuri Radix (BR) and vinegar-baked Bupleuri Radix (VBBR). Furthermore, behavioral studies along with biochemical assessments, as well as 1H NMR analysis of the hippocampus and liver were employed to systematically assess the pathology of depression and therapeutic effects of BR and VBBR using a rat model of chronic unpredictable mild stress (CUMS)-induced depression. Rats were divided into 5 groups (n = 6 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 15 mg/kg), (4) CUMS + BR group (stress plus Bupleuri Radix treatment at the dose of 10 g/kg), and (5) CUMS + VBBR group (stress plus vinegar-baked Bupleuri Radix treatment at the dose of 10 g/kg). The CUMS stress procedure lasted for 6 weeks, and drugs were administered orally once daily during the last 3 weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 30 |
Tissue | Central; Brain; Hippocampus; Peripheral; Liver; Liver; |
Platform | NMR; NMR: Brucker AV 600 MHz (Brucker, Germany) superconducting Fourier transform NMR spectrometer; |
PMID | |
DOI | |
Citation | Lei T, Wang Y, Li M, et al. A comparative study of the main constituents and antidepressant effects of raw and vinegar-baked Bupleuri Radix in rats subjected to chronic unpredictable mild stress. RSC Adv 2017;7(52),32652-63. |
Metabolite | myo-Inositol; Glycerol; L-Phenylalanine; L-Leucine; Glycine; L-Lactic acid; L-Alanine; |
