Study M1064
Study name | Makova M 2023 |
Title | Mirtazapine modulates Glutamate and GABA levels in the animal model of maternal depression. MRI and 1H MRS study in female rats |
Overall design | The aim of this study was to determine whether maternal depression induced by chronic unpredictable stress (CUS) in the pre-gestational period in female rats would be evidenced by neurometabolic changes in the hippocampal region of the brain. Female Wistar rats were divided into the following 4 groups: (1) control group (non-stress, with vehicle treatment), (2) mirtazapine group (non-stress, with mirtazapine treatment), (3) CUS group (stress, with vehicle treatment), and (4) CUS + mirtazapine group (stress, with mirtazapine treatment). Animals in the stress groups were exposed to CUS for a total of three weeks. One week after the end of the stress procedure, females were mated with males. Mirtazapine was administered orally to pregnant rats from day 10 of gestation until weaning on day 21 post-partum, at the dose of 10 mg/kg once per day. Forced swimming test was performed on day 76 post-partum. Proton magnetic resonance spectroscopy was performed focused on the hippocampus on days 82-86 post-partum (n = 6 in each group). Metabolite quantification was reported relative to total creatine as the reference peak. |
Study Type | Type1; Type2; Type3; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; Healthy individuals; Healthy individuals; Healthy individuals; |
Criteria for depression | Forced swimming test |
Sample size | 22 |
Tissue | Central; Brain; Hippocampus; |
Platform | MRS; MRS: 200/300 horizontal bore MR scanner; |
PMID | |
DOI | |
Citation | Makova M, Kasparova S, Tvrdik T, et al. Mirtazapine modulates Glutamate and GABA levels in the animal model of maternal depression. MRI and 1H MRS study in female rats. Behav Brain Res. 2023 Mar 28;442:114296. |
Metabolite | GABA/(Creatine and Phosphocreatine) ratio; (Glutamate and Glutamine)/(Creatine and Phosphocreatine) ratio; |
