Study M1041
Study name | Jia XX 2023 |
Title | Mitochondrial transplantation ameliorates hippocampal damage following status epilepticus |
Overall design | The aim of this study was to explore whether treatment with exogenous mitochondria would ameliorate status epilepticus-induced hippocampal damage including neuronal loss, gliosis, cognitive deficit and emotional disorders. C57BL/6J mice were divided into the following 3 groups: (1) control group, (2) pilocarpine group, and (3) pilocarpine + mitochondrial transplantation group. Pilocarpine (320 mg/kg) was used to induced status epilepticus in mice. Exogenous fresh mitochondria isolated from hippocampus of normal mice were administered once by the tail vein injection (1 mg/kg) 1 hour after status epilepticus. The behavioral tests were implemented 7-10 days after status epilepticus. The immobility time of the pilocarpine group in the tail suspension test was significantly elevated compared to the control group, and mitochondrial transplantation decreased the immobility time. The metabolites underlying mitochondrial transplantation were determined using LC-MS-based metabonomics (n = 6/group). |
Study Type | Type1; Type2; |
Data available | |
Organism | Mouse; C57BL/6J mouse; |
Categories of depression | Animal model; Other animal model; Other animal model; |
Criteria for depression | Tail suspension test |
Sample size | 18 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: UHPLC system (Vanquish UHPLC, Thermo) with quadrupole time-of-flight system (AB Sciex TripleTOF 6600); |
PMID | |
DOI | |
Citation | Jia X, Wang Q, Ji J, et al. Mitochondrial transplantation ameliorates hippocampal damage following status epilepticus. Animal Model Exp Med. 2023 Feb;6(1):41-50. |
Metabolite | Dimethylglycine; Indoxyl sulfate; 3-Phosphoglyceric acid; Fructose 1,6-bisphosphate; Glyceraldehyde 3-phosphate; L-Acetylcarnitine; Methylmalonic acid; D-Ribose 5-phosphate; PC(38:6); SM(d18:1/16:0); SM(d18:1/18:0); Mannose 6-phosphate; Epsilon-caprolactam; PE(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)); Pyridoxal; Thioetheramide-PC; PC(18:0/18:1(9Z)); SM(d18:1/18:1(9Z)); DG(18:0/20:4(5Z,8Z,11Z,14Z)/0:0); PE(16:0/18:1(9Z)); PG(44:12); Asiatic acid; Polygalic acid; Isopentenyl pyrophosphate; 4-benzofuranethanamine, 2,3-dihydro-.alpha.-methyl-; Trehalose; Gingerol; D-myo-Inositol 1,4-bisphosphate; Methylprednisolone hemisuccinate; hydroxyisovaleroyl carnitine; 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid; PG(42:10); 5,8,11,14-eicosatetraenamide, n-(4-hydroxy-2-methylphenyl)-, (5z,8z,11z,14z)-; Cer(d18:0/14:0); 2-Dhahpa; Dronedarone; 2-arachidonoyl-1-stearoyl-sn-glycero-3-phosphoethanolamine; |
