Study M1040
Study name | Hao ZK 2023 |
Title | Positive mood-related gut microbiota in a long-term closed environment: a multiomics study based on the "Lunar Palace 365" experiment |
Overall design | The aim of this study was to investigate the effect of potential psychobiotics (including Bacteroides uniformis, Roseburia inulinivorans, and Eubacterium rectale) on host mood in the chronic unpredictable mild stress (CUMS)-induced depression- and anxiety-like rats. Sprague-Dawley rats were divided into the following 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 10 mg/kg), (4) CUMS + Roseburia inulinivorans group (stressor plus Roseburia inulinivorans treatment at the dose of 10^9 CFU), (5) CUMS + Bacteroides uniformis group (stressor plus Bacteroides uniformis treatment at the dose of 10^9 CFU), and (6) CUMS + Eubacterium rectale group (stressor plus Eubacterium rectale treatment at the dose of 10^9 CFU). The CUMS stress procedure lasted for 4 weeks, and drugs were administered via intragastric once per day for 4 weeks after the model building period. Fecal sample was used for short-chain fatty acid measurement by gas chromatography-mass spectrometry. Brain sample was used for neurotransmitter measurement by ultra-performance liquid chromatography-mass spectrometry. Six samples per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Forced swimming test |
Sample size | 36 |
Tissue | Peripheral; Faece; Faece; Central; Brain; Brain; |
Platform | MS-based; GC-MS: Thermo TRACE 1310-ISQ LT; MS-based; LC-MS: ACQUITY ultra-performance liquid chromatography-MS (Waters Corp., Milford, MA, USA); |
PMID | |
DOI | |
Citation | Hao Z, Meng C, Li L, et al. Positive mood-related gut microbiota in a long-term closed environment: a multiomics study based on the "Lunar Palace 365" experiment. Microbiome. 2023 Apr 24;11(1):88. |
Metabolite | L-Glutamine; 5-Hydroxyindoleacetic acid; |
