Study M103
Study name | de Sousa Rodrigues ME 2017 |
Title | Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice |
Overall design | The aim of the present study was to assess the effects of mild chronic psychological stress on metabolic, inflammatory, and behavioral profiles in a mouse model of diet-induced obesity. C57Bl/6 male mice underwent a combination of high-fat high-fructose diet (HFHF) and two weeks of chronic psychological stress. MetS-related conditions were assessed using untargeted plasma metabolomics. Mice were divided into 4 groups: (1) control group (control diet and no stress, n = 14), (2) stress group (control diet and predatory stress, n = 15), (3) high-fat high-fructose group (no stress, with high-fat high-fructose diet, n = 14), and (4) stress + high-fat high-fructose group (predatory stress and high-fat high-fructose diet, n = 15). To test for alterations in metabolism due to HFHF diet and its interaction with the stress exposure, untargeted HRM profiling was performed using plasma collected on week 8 to evaluate the effect of HFHF diet on the plasma metabolome. Sample was collected from n = 8 - 13 mice/group. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Mouse; C57BL/6 mouse; |
Categories of depression | Animal model; Social defeat model; Social defeat model; |
Criteria for depression | Female urine sniffing test, sociability test |
Sample size | 58 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: hydrophilic interaction liquid chromatography (HILIC) and C18 chromatography with acetonitrile/formic acid gradient interfaced to Q-Exactive HF high-resolution mass spectrometer (Thermo Scientific); |
PMID | |
DOI | |
Citation | de Sousa Rodrigues ME, Bekhbat M, Houser MC, et al. Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice. Brain Behav Immun 2017;59:158-72. |
Metabolite |
