Study M1027
Study name | Huang MN 2023 |
Title | Gut microbiota-SCFAs-brain axis associated with the antidepressant activity of berberine in CUMS rats |
Overall design | The aim of this study was to explore the anti-depressant mechanisms of berberine involving the association of the gut microbiota, neurotransmitters, BDNF, and short-chain fatty acids (SCFAs) in chronic unpredictable mild stress (CUMS)-induced depressive rats. Wistar rats were divided into the following 5 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stressor plus fluoxetine treatment at the dose of 7.5 mg/kg), (4) CUMS + low dose of berberine group (stressor plus berberine treatment at the dose of 50 mg/kg), and (5) CUMS + high dose of berberine group (stressor plus berberine treatment at the dose of 100 mg/kg). The CUMS stress procedure lasted for 5 weeks, and drugs were administered via intragastric once per day during the last 2 weeks of the model building period. The concentrations of fecal SCFAs were analyzed by GC-MS targeted metabolomics. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 50 |
Tissue | Peripheral; Faece; Faece; |
Platform | MS-based; GC-MS: Agilent 7890A/5975C gas mass spectrometer (Agilent, USA); |
PMID | |
DOI | |
Citation | Huang M, He Y, Tian L, et al. Gut microbiota-SCFAs-brain axis associated with the antidepressant activity of berberine in CUMS rats. J Affect Disord. 2023 Mar 15;325:141-150. |
Metabolite |
