Study M1020
Study name | Bian XQ 2022b |
Title | Identification of proline, 1-pyrroline-5-carboxylate and glutamic acid as biomarkers of depression reflecting brain metabolism using carboxylomics, a new metabolomics method |
Overall design | In this study, a sensitive 5-(diisopropylamino) amylamine derivatization coupled with UHPLC-Q-TOF/MS was used to profile carboxylic acids in serum samples of patients with depression. Then, the serum, cerebrospinal fluid (CSF), and hippocampus from chronic mild unpredictable stress (CUMS) model of depression were used to further confirm the results. Sprague-Dawley rats were randomly divided into two group (n = 6 in each group): (1) control group, and (2) CUMS group. Sucrose preference test, tail suspension test, and forced swim test were examined after stress. Serum, CSF, and hippocampus of each rat were collected for metabolomics analysis. Glycine, 1-pyrroline-5-carboxylate, arginine, aspartic acid, ornithine, proline, glutamic acid, and glutathione were extracted. The difference with P < 0.05 was considered as statistical significance. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test, tail suspension test, forced swim test |
Sample size | 12 |
Tissue | Peripheral; Blood; Serum; Central; Cerebrospinal fluid; Cerebrospinal fluid; Central; Brain; Hippocampus; |
Platform | MS-based; LC-MS: Agilent 1290 Infinity LC system (UHPLC, Santa Clara) with Agilent 6545 Q-TOF/MS system; |
PMID | |
DOI | |
Citation | Bian X, Zhou N, Zhao Y, et al. Identification of proline, 1-pyrroline-5-carboxylate and glutamic acid as biomarkers of depression reflecting brain metabolism using carboxylomics, a new metabolomics method. Psychiatry Clin Neurosci. 2023 Apr;77(4):196-204. |
Metabolite |
