Study M099

Study name

Abildgaard A 2017

Title

Probiotic treatment reduces depressive-like behaviour in rats independently of diet

Overall design

The aim of this study was to investigate whether the habitual diet may interact with the effect of probiotics on depression-related behaviour and further examined some potentially involved mechanisms underlying the microbe-mediated behavioural effects. A non-targeted plasma metabolomics analysis was applied using Ultrahigh performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS). Rats were divided into 4 groups (n = 10 in each group): (1) control group (no high-fat diet and no drug), (2) probiotics group (no high-fat plus multi-species probiotic formulation), (3) high-fat diet group (high-fat diet and no drug), and (4) high-fat diet + probiotics group (high-fat diet and multi-species probiotic formulation). The probiotics consisted of eight bacterial strains (B. bifidum W23, B. lactis W52, L. acidophilus W37, L. brevis W63, L. casei W56, L. salivarius W24, Lc. Lactis W19, Lc. Lactis W58; "Ecologic Barrier", Winclove Probiotics BV, The Netherlands) in a carrier matrix of maize starch, maltodextrins and vegetable protein. Each cage received a bottle containing 4.5 g (2.5 x 109 CFU/g) of freeze-dried powder dissolved in 30 mL of tap water. The bottles were administered daily between four and six pm and completely emptied by the animals during the night. The high-fat diet procedure lasted for 10 weeks, and drugs were administered during the last 5 weeks.

Study Type

Type3;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Healthy individuals; Healthy individuals; Healthy individuals;

Criteria for depression

Forced swimming test

Sample size

40

Tissue

Peripheral; Blood; Plasma;

Platform

MS-based; LC-MS: Ultrahigh performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS);

PMID

28259042

DOI

10.1016/j.psyneuen.2017.02.014

Citation

Abildgaard A, Elfving B, Hokland M, et al. Probiotic treatment reduces depressive-like behaviour in rats independently of diet. Psychoneuroendocrinology 2017;79:40-8.

Metabolite

Indole-3-propionic acid;

Indoleacrylic acid;

N-delta-acetylornithine;