Study M086

Study name

Park DI 2016

Title

Purine and pyrimidine metabolism: convergent evidence on chronic antidepressant treatment response in mice and humans

Overall design

DBA/2J mice were treated for 28 days with paroxetine and assessed their behavioral response with the forced swim test (FST). Paroxetine-treated long-time floating (PLF) and paroxetine-treated short-time floating (PSF) groups were stratified as proxies for drug non-responder and responder mice, respectively. Proteomics and metabolomics profiles of PLF and PSF groups were acquired for the hippocampus and plasma to identify molecular pathways and biosignatures that stratify paroxetine-treated mouse sub-groups. All the mice were divided into 2 groups (n = 8 in each group): (1) control group (vehicle), (2) paroxetine group (paroxetine treatment at the dose of 5 mg/kg twice a day). Paroxetine group were divided into responder (short-time floating, PSF) and non-responder (long-time floating, PLF) mice according to their forced swim test floating time. Five samples per group were used for metabonomic analysis.

Study Type

Type5;

Data available

Unavailable

Organism

Mouse; DBA/2J mouse;

Categories of depression

Healthy individuals; Healthy individuals; Healthy individuals;

Criteria for depression

Forced swimming test, female urine sniffing test

Sample size

10

Tissue

Central; Brain; Hippocampus;

Central; Brain; Prefrontal cortex;

Peripheral; Blood; Plasma;

Platform

MS-based; LC-MS: Prominence UFLC high-performance liquid chromatography system (Shimadzu, Columbia, MD, USA) with 5500 QTRAP triple quadrupole mass spectrometer (AB/SCIEX, Framingham, MA, USA);

PMID

27731396

DOI

10.1038/srep35317

Citation

Park DI, Dournes C, Sillaber I, et al. Purine and pyrimidine metabolism: convergent evidence on chronic antidepressant treatment response in mice and humans. Sci Rep 2016;6:35317.

Metabolite

myo-Inositol;

Citric acid;

Isocitric acid;

Flavone;

Phosphorylcholine;

Carbamoyl phosphate;

(S)-2-Methylmalate;

Sarcosine;

Glucosamine 6-phosphate;

Thymidine;

Citraconic acid;

L-Dihydroorotic acid;

Folic acid;

Geranyl-PP;

Glucosamine;

Orotic acid;