Study M071
Study name | Zhao J 2015 |
Title | Metabolomic identification of biochemical changes induced by fluoxetine and imipramine in a chronic mild stress mouse model of depression |
Overall design | Metabolomics was applied to a C57BL/6N mouse model of chronic unpredictable mild stress (CMS). Such mice were treated with two antidepressants from different categories: fluoxetine and imipramine. Metabolic profiling of the hippocampus was performed using gas chromatography-mass spectrometry analysis. Mice were randomly divided into 4 groups (n = 7 in each group): (1) control group, (2) CUMS group, (3) CUMS + fluoxetine group (stress plus fluoxetine treatment at the dose of 20 mg/kg), and (4) CUMS + imipramine group (stress plus imipramine treatment at the dose of 20 mg/kg). The CUMS stress procedure lasted for 4 weeks, and drugs were administered by intraperitoneal injection once a day during the last 2 weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Mouse; C57BL/6N mouse; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Forced swimming test |
Sample size | 28 |
Tissue | Central; Brain; Hippocampus; |
Platform | MS-based; GC-MS: Hewlett-Packard (HP) GC system 6890 Series equipped with 5973 Mass Selective Detector (MSD) system; |
PMID | |
DOI | |
Citation | Zhao J, Jung YH, Jang CG, et al. Metabolomic identification of biochemical changes induced by fluoxetine and imipramine in a chronic mild stress mouse model of depression. Sci Rep 2015;5:8890. |
Metabolite | myo-Inositol; Oleic acid; Stearic acid; Palmitic acid; N-Acetyl-L-aspartic acid; L-Valine; L-Leucine; L-Glutamic acid; Glycine; L-Glutamine; |
