Study M063
Study name | Tian JS 2015b |
Title | Investigation on the antidepressant effect of sea buckthorn seed oil through the GC-MS-based metabolomics approach coupled with multivariate analysis |
Overall design | With the objective of finding potential biomarkers of the therapeutic response of sea buckthorn seed oil in chronic unpredictable mild stress (CUMS) rats, urine metabolomics based on gas chromatography-mass spectrometry (GC-MS) coupled with multivariate analysis was applied. Rats were randomly divided into 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + high dose of the sea buckthorn seed oil group (stress plus sea buckthorn seed oil at the dose of 1.40 g/kg), (4) CUMS + middle dose of the sea buckthorn seed oil group (stress plus sea buckthorn seed oil at the dose of 0.7 g/kg), (5) CUMS + low dose of the sea buckthorn seed oil group (stress plus sea buckthorn seed oil at the dose of 0.35 g/kg), and (6) CUMS + venlafaxine group (stress plus venlafaxine hydrochloride 50 mg/kg). Six samples per group were used for metabonomic analysis. The CUMS stress procedure lasted for 4 weeks, and drugs were administered via gastric intubation during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 36 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; GC-MS: Gas chromatograph coupled with a PolarisQ Ion Trap mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA); |
PMID | |
DOI | |
Citation | Tian JS, Liu CC, Xiang H, et al. Investigation on the antidepressant effect of sea buckthorn seed oil through the GC-MS-based metabolomics approach coupled with multivariate analysis. Food Funct 2015;6(11):3585-92. |
Metabolite |
