Study M055
Study name | Chen JL 2015 |
Title | 1H NMR-based metabolic profiling of liver in chronic unpredictable mild stress rats with genipin treatment |
Overall design | In this study, the metabolic profiles of aqueous and lipophilic extracts in liver of the chronic unpredictable mild stress (CUMS)-induced rat with genipin treatment were investigated using proton nuclear magnetic resonance (1H NMR) spectroscopy coupled with multivariate data analysis. Rats were weighed and randomly divided into 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + venlafaxine group (stress plus venlafaxine treatment at the dose of 50 mg/kg), (4) CUMS + high dose of genipin group (stress plus genipin treatment at the dose of 100 mg/kg), (5) CUMS + middle dose of genipin group (stress plus genipin treatment at the dose of 50 mg/kg body weight), and (6) CUMS + low dose of genipin group (stress plus genipin treatment at the dose of 25 mg/kg body weight). Six samples per group were used for metabonomic analysis. The CUMS procedure was conducted for 4 weeks, whereas drug administration was performed daily via gastric intubation during the last two weeks of the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 36 |
Tissue | Peripheral; Liver; Liver; |
Platform | NMR; NMR: Bruker 600-MHz AVANCE III NMR spectrometer (Bruker, Germany); |
PMID | |
DOI | |
Citation | Chen JL, Shi BY, Xiang H, et al. 1H NMR-based metabolic profiling of liver in chronic unpredictable mild stress rats with genipin treatment. J Pharm Biomed Anal 2015;115:150-8. |
Metabolite | Glycerol; |
