Study M045
Study name | Li J 2014 |
Title | Peripheral blood mononuclear cell-based metabolomic profiling of a chronic unpredictable mild stress rat model of depression |
Overall design | In this study, PBMCs were obtained from a chronic unpredictable mild stress (CUMS) rodent model of depression. A gas chromatography-mass spectrometry (GC-MS) metabolomic approach coupled with principal component analysis (PCA) and open partial least-squares discriminant analysis (OPLS-DA) statistical analysis was used to detect differential metabolites in PBMCs of depressed rats. Rats were divided into 2 groups: (1) control group and (2) CUMS group. Nine samples per group were used for metabonomic analysis. The CUMS stress procedure lasted for 4 weeks. |
Study Type | Type1; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 18 |
Tissue | Peripheral; Blood; Peripheral blood mononuclear cell; |
Platform | MS-based; GC-MS: Agilent 7890A/5975C GC-MS System (Agilent Technologies Inc., USA); |
PMID | |
DOI | |
Citation | Li J, Tang G, Cheng K, et al. Peripheral blood mononuclear cell-based metabolomic profiling of a chronic unpredictable mild stress rat model of depression. Mol Biosyst 2014;10 (11):2994-3001.; Li J, Zhang SX, Wang W, et al. Potential antidepressant and resilience mechanism revealed by metabolomic study on peripheral blood mononuclear cells of stress resilient rats. Behav Brain Res 2017;320:12-20. |
Metabolite | myo-Inositol; Myristic acid; L-Glutamic acid; Glycine; L-Aspartic acid; D-Fructose; Creatinine; Serotonin; Ethanolamine; Beta-Alanine; Glycolic acid; Adenosine monophosphate; Alpha-Tocopherol; |
