Study M039
Study name | Chen SL 2014 |
Title | Effect of Allium macrostemon on a rat model of depression studied by using plasma lipid and acylcarnitine profiles from liquid chromatography/mass spectrometry |
Overall design | The present study aimed to investigate the anti-depressive effect of the traditional Chinese medicine Allium macrostemon (A.M.) in a rat model of depression induced by exposure to chronic immobilization stress. Lipid and acylcarnitine metabolism were set into the focus of this study due to their key role in the pathogenesis of depression. Plasma lipid profiling was performed by ultra fast liquid chromatography/ion trap-time of flight mass spectrometry. Ultra-high performance liquid chromatography/triple quadrupole mass spectrometry was used to characterize the plasma acylcarnitine profile. Rats were divided into 3 groups: (1) control group (n = 12), (2) CRS group (chronic immobilization stress, n = 12), and (3) CRS + A.M. group (chronic immobilization stress plus A.M. treatment at the dose of 10 mL/kg, n = 11). Rat in the CRS group or CRS + A.M. group was kept in a restraint box for 6 h every day, and the animal experiments lasted for 6 weeks. A.M. was administered once per day by intragastric during the model building period. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Wistar rat; |
Categories of depression | Animal model; Chronic restraint stress model; Chronic restraint stress model; |
Criteria for depression | Tail suspension test |
Sample size | 35 |
Tissue | Peripheral; Blood; Plasma; |
Platform | MS-based; LC-MS: Ultra fast liquid chromatography system coupled with ion trap time-of-flight mass spectrometry (UFLC/IT-TOF MS, Shimadzu, Kyoto, Japan); MS-based; LC-MS: ultra-high performance liquid chromatography system coupled with triple quadrupole mass spectrometry (LC/MS QQQ 6460, Agilent, Santa Clara, CA, USA); |
PMID | |
DOI | |
Citation | Chen S, Wei C, Gao P, et al. Effect of Allium macrostemon on a rat model of depression studied by using plasma lipid and acylcarnitine profiles from liquid chromatography/mass spectrometry. J Pharm Biomed Anal 2014;89:122-9. |
Metabolite | LysoPC(18:1); LysoPC(18:2); LysoPC(20:1); LysoPC(O-16:2); LysoPC(O-18:3); Carnitine C10:1; Carnitine C12:0; Carnitine C14:2; Carnitine C14:1; Carnitine C14:0; Carnitine C16:2; Carnitine C16:1; Carnitine C16:0; Linoelaidylcarnitine; Oleoylcarnitine; Stearoylcarnitine; Carnitine C20:2; Carnitine C20:1; Carnitine C20:0; Carnitine C14:0-OH; Carnitine C16:1-OH; Carnitine C16:0-OH; Carnitine C18:2-OH; Carnitine C18:1-OH; PC(32:1); PC(36:4); PC(36:5); |
