Study M023

Study name

Fenli S 2013

Title

Biochemical mechanism studies of venlafaxine by metabonomic method in rat model of depression

Overall design

The aim of this study was to study the biochemical mechanism of venlafaxine through determining the metabolism of monoamine neurotransmitters in brain tissues of rat model of depression after administration of venlafaxine using metabonomic method. 5-HT, NE, DA and their metabolites were determined in rat brain tissues by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) following chronic administration of different venlafaxine doses and saline solution for 14 days. Rats were divided into 6 groups (n = 10 in each group): (1) control group, (2) CUMS group, (3) CUMS + low dose of venlafaxine group (stress plus venlafaxine treatment at the dose of 8 mg/kg), (4) CUMS + middle dose of venlafaxine group (stress plus venlafaxine treatment at the dose of 16 mg/kg), (5) CUMS + high dose of venlafaxine group (stress plus venlafaxine treatment at the dose of 32 mg/kg), and (6) CUMS + fluoxetine group (stress plus venlafaxine treatment at the dose of 2 mg/kg). The CUMS stress procedure lasted for 3 weeks, and drugs were intragastric administered daily for 2 weeks after the model building period.

Study Type

Type1;

Type2;

Data available

Unavailable

Organism

Rat; Sprague-Dawley rat;

Categories of depression

Animal model; Chronic mild stress model; Chronic mild stress model;

Criteria for depression

Sucrose preference test

Sample size

56

Tissue

Central; Brain; Brain;

Platform

MS-based; LC-MS: Waters Acquity Ultra Performance LC system (Waters, Milford, MA, USA) with a Micromass Quattro Premier XE tandem quadruple mass spectrometer (Waters, Manchester, UK) equipped with electrospray ionization (ESI);

PMID

23329522

DOI

N/A

Citation

Fenli S, Feng W, Ronghua Z, et al. Biochemical mechanism studies of venlafaxine by metabonomic method in rat model of depression. Eur Rev Med Pharmacol Sci 2013;17(1):41-8.

Metabolite

5-Hydroxyindoleacetic acid;

Serotonin;

Norepinephrine;

5-HIAA/5-HT ratio;

MHPG/NE ratio;