Study M004
Study name | Dai YT 2010 |
Title | Metabolomics study on the anti-depression effect of xiaoyaosan on rat model of chronic unpredictable mild stress |
Overall design | In this study, a GC-MS-based urinary metabolomics method was applied to evaluate the efficacy of xiaoyaosan on rat model of chronic unpredictable mild stress. Rats were randomly divided into 6 groups (n = 8 in each group): (1) control group (no stress and no drug), (2) CUMS group (stress plus pure water), (3) CUMS + high dose of xiaoyaosan group (stress plus xiaoyaosan at the dose of 13.50 g/kg), (4) CUMS + middle dose of xiaoyaosan group (stress plus xiaoyaosan at the dose of 6.75 g/kg), (5) CUMS + low dose of xiaoyaosan group (stress plus xiaoyaosan at the dose of 3.38 g/kg), and (6) CUMS + amitriptyline group (stress plus amitriptyline 10 mg/kg). The CUMS stress procedure lasted for 3 weeks, and drugs were administered via gastric intubation during the model building period. Six sample per group were used for metabonomic analysis. |
Study Type | Type1; Type2; |
Data available | Unavailable |
Organism | Rat; Sprague-Dawley rat; |
Categories of depression | Animal model; Chronic mild stress model; Chronic mild stress model; |
Criteria for depression | Sucrose preference test |
Sample size | 36 |
Tissue | Peripheral; Urine; Urine; |
Platform | MS-based; GC-MS: trace gas chromatograph coupled with a PolarisQ Ion Trap mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA); |
PMID | |
DOI | |
Citation | Dai Y, Li Z, Xue L, et al. Metabolomics study on the anti-depression effect of xiaoyaosan on rat model of chronic unpredictable mild stress. J Ethnopharmacol 2010;128(2):482-9. |
Metabolite | Palmitic acid; Glycine; L-Alanine; Hippuric acid; Adipic acid; |
